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scoC [2019-07-11 10:17:55]
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scoC [2019-07-11 10:17:55]

transcriptional repressor of genes expressed in the transition phase
Locus
BSU09990
Isoelectric point
5.19
Molecular weight
23.57 kDa
Protein length
203 aa Sequence Blast
Gene length
612 bp Sequence Blast
Function
transition state regulator
Product
transcriptional repressor (MarR family)
Essential
no
Synonyms
hpr,catA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,073,106 → 1,073,717

The protein

Modification

  • phosphorylated on Arg-3 PubMed
  • Effectors of protein activity

  • activity is inhibited in an unknown way by PrkA (possibly phosphorylation) PubMed
  • Structure

    Additional information

  • information on binding sites can be found in the PRODORIC2 database
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • AbrB: activation, PubMed, in AbrB regulon
  • SenS: positive regulation, in SenS regulon
  • SalA: negative regulation, in SalA regulon
  • CodY: negative regulation, in CodY regulon
  • Regulation

  • expressed upon transition into the stationary phase (AbrB) PubMed
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    Biological materials

    Mutant

  • 1A918 ( scoC::erm trpC2 leuC7), PubMed, available at the Bacillus Genetic Stock Center
  • BKE09990 (ΔscoC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
  • BKK09990 (ΔscoC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
  • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • References

    Reviews

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    Original publications

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